Quantification of Cyclic Electron Flow in Spinach Leaf Discs
We quantified the photosynthetic cyclic electron flux (CEF) around Photosystem Ⅰ as the difference between the total electron flux through PS Ⅰ (ETR1) and the linear electron flux through both photosystems. Both measurements were made in the whole tissue of spinach leaf discs illuminated in the same geometry and in CO2-enriched air to suppress photorespiration. (1) CEF was negligibly small below 300 umol photons m-2 s-1. Above this irradiance, CEF increased approximately linearly up to the highest irradiance used (1,900 umol photons m-2s-1). (2) CEF at a fixed irradiance of 980 μmol m-2 s-1 increased by a factor of almost 3 as the temperature was increased from 5 ℃ to 40 ℃. It did not decline, even when the linear electron flux decreased at high temperatures. (3) Antimycin A, at a high concentration, decreased CEF to about 10％ of the control value without affecting the linear electron flux. This method appears to be reliable for quantifying CEF nonintrusively. By contrast, estimation of the linear electron flux from chlorophyll fluorescence overestimated CEF in the above treatments.
Antimycin A Cyclic electron flow Linear electron flow P700 Photosystem Ⅰ
Jiancun Kou Shunichi Takahashi Riichi Oguchi Murray R Badger Wah Soon Chow
North-West Agricultural & Forestry University, Yangling, Shaanxi 712100, China Division of Plant Sci Division of Plant Science, Research School of Biology, College of Medicine, Biology and Environment,
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
271-274
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）