Expression of PEPC Gene, Lipid Content and Photosynthesis in Anabaena 7120
The aim of this work was to prepare a material for biodiesel. Filamentous cyanobacterium Anabaena 7120 was mutated by DNA recombination technique. Anti and sense vectors, harbering phosphoenolpyruvate carboxylase (PEPC) gene fragment pepcA, were constructed and transformed Anabaena 7120. The downregulating mutant decreased PEPC activity to 12.9％ of that in wild type cells, and its lipid content elevated 54.7％ of that in wild type cells. The lipid content may increase to 54.15％ of dry weigh. The mutant elevated net photosynthetic activity 51％-77％ higher than that in wild type cells, and the activity in BG11(-N) medium was higher than in BG11(+N) medium. Also, its tolerance to higher light intensity and temperature was enhanced. From these data, the mutant contained more lipid, grew fast, tolerated high light intensity and temperature, harvested easily and the species of cyanobacterium was no toxic. So, using the mutant as a feedstock for biodiesel production may reduce the cost.
PEPC (phosphoenolpyruvate carboxylase) Gene expression Lipid content Photosynthesis Anabaena (Nostoc) 7120 Biodiesel
Lijun Hou Xiaohui Jia Dingji Shi Xuekui Wang
School of Marine Sciences & Engineering, Tianjin University of Sciences & Technology, Tianjin 300457 School of Marine Sciences & Engineering, Tianjin University of Sciences & Technology, Tianjin 300457
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
335-338
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）