Regulation of the Carbon and Nitrogen Balance by a Plastidic Invertase in Arabidopsis
Since the photosynthetic apparatus contains a massive amount of nitrogen in plants, the regulation of its development by sugar signals is important to the maintenance of the carbon-nitrogen balance. Here, we isolated an Arabidopsis mutant (sicy-192) whose cotyledon greening was inhibited by treatments with sugars, such as sucrose, glucose, and fructose. In the mutant, the gene encoding plastidic alkaline/neutral (A/N) invertase (INV-E) was pointmutated at codon 294, with Tyr substituted for Cys (C294Y). Interestingly, the greening of cotyledons in the knockout-INV-E lines was not inhibited by treatment with the sugars. The mutant INV-E was more stable than the wild-type INV-E. On treatment with sucrose, the expression of photosynthesis-related genes was weaker in seedlings of mutant plants than wild-type seedlings, while the activity of nitrate reductase was stronger in the mutant plants than wild-type plants. These findings suggest that Cys294 of INV-E is associated with the development of the photosynthetic apparatus and the assimilation of nitrogen in Arabidopsis seedlings to control the ratio of sucrose content to hexose content. The transcript and protein levels of plastid-encoded genes were lower in the sicy-192 mutants than the wild-type plants, suggesting that sugar metabolism through INV-E promotes plastid signaling to regulate the carbon-nitrogen balance.
Invertase Carbon/nitrogen (C/N) balance Greening Arabidopsis
Takanori Maruta Kumi Otori Tomoki Tabuchi Noriaki Tanabe Masahiro Tamoi Shigeru Shigeoka
Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CRE Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CRE
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
344-347
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）