Blue-Native Page Analysis Validates Heterogeneity in the Thylakoids of Synechocystis 6803
Thylakoids from mature chloroplasts of higher plants show distinct ultra structural differentiation into grana and stroma lamellae where the photosystems, PSⅡ and PSⅠ, are differentially distributed. The thylakoids from cyanobacteria do not show such differentiation, even if they have both PSⅡ and PSⅠ as components of electron transport like higher plants. Recently we have demonstrated that the thylakoids from cell free extracts of Synechocystis 6803 can be fractionated using differential ultracentrifugation at 40,000 x g (40 k), 90,000 x g (90 k) and 150,000 x g (150 k). These native thylakoid fractions showed biochemical as well as proteomic heterogeneity. We have analyzed these fractions using Blue-Native (BN) PAGE followed by ESI-MS/MS or MALDIMS. The 1D BN gels showed protein super-complexes bands ranging from ～200～900 kDa. The protein supercomplexes from 1-D gels were further separated into individual subunits in second dimension on the denaturing tricine SDS-PAGE. 44 differing bands from 1-D gels and 38 differing protein spots form 2-D gels were selected for identification. Our results showed that there were qualitative and quantitative differences in the pigment-protein complexes among the three fractions validating the hypothesis of heterogeneity in thylakoids of Synechocystis 6803.
Blue-Native PAGE Heterogeneity Synechocystis 6803 Thylakoid membranes
Rachna Agarwal Andrea Matros Michael Melzer Hans-Peter Mock Jayashree Krishna Sainis
Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai, India Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben (IPK), Germany
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
385-388
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）