State Transition Mechanism in Arabidopsis Thaliana: Biophysical and Proteomic Studies
The redox state of plastoquinone (PQ) pool is the incipient signal in the signal transduction pathway of state transition mechanism, shifting from state I to state Ⅱ and vice versa. The redox state of the Qa, the primary acceptor of Photosystem Ⅱ (PSⅡ) and the PQ pool are easily monitored by the OJIP fast fluorescence transients. The OJIP fast Chi a fluorescence transient studies revealed that in state Ⅱ, there was reduction in maximal fluorescence which could be due to decreased antennae size of PSⅡ. The same changes were not observed in Stn7 mutant lacking thylakoid kinase which phosphorylates light harvesting complex (LHC) II. The phosphorylated LHCII is associated with PSⅠ under state Ⅱ, condition. The redox state of PQ pool is signal for the kinase to phosphorylate/dephosphorylate major LHCII of PSⅡ. The 2-D electrophoresis results showed that LHCII is resolved into 3 spots in state I. However, in state Ⅱ this has been resolved into 4 spots. However, Stn7 mutant there was no change of 2D spots in state Ⅱ. The additional spot is yet to be investigated.
2D electrophoresis Arabidopsis thaliana Chl a fluorescence Photosystems State transition
Sreedhar Nellaepalli Ottó Zsiros László Kovács Yadavalli Verikateswarlu Mekala Nageswara Rao Prasanna Mohanty Rajagopal Subramanyam
Department of Biochemistry, University of Hyderabad, Hyderabad, 500046 India Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hunga Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046 In Department of Biochemistry, University of Hyderabad, Hyderabad, 500046 India Department of Plant Sci
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
398-401
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）