Solution Structure and Physiological Requirements for Psb27 in Synechocystis sp. PCC 6803
Photosystem Ⅱ (PS Ⅱ) is a membrane-embedded protein complex that has a highly regulated assembly pathway. A number of proteins that interact transiently with PS Ⅱ have been identified. One of these proteins Psb27, is a cyanobacterial lipoprotein that binds to inactive PS Ⅱ monomers and is involved in the biogenesis of the active PS Ⅱ dimer. We have solved the solution structure of Psb27 and have identified a group of conserved residues that potentially form an interaction with PS Ⅱ Mabbitt PD, Rautureau GJP, Day CL, Wilbanks SM, Eaton-Rye JJ, Hinds MG (2009) Biochemistry 48: 8771-8773. Despite the conservation of Psb27 across plants and cyanobacteria, APsb27 Synechocystis sp. PCC 6803 cells lack a phenotype when grown under standard laboratory conditions. To assess Psb27 function, we have identified conditions where the APsb27 mutant cannot grow photoautotrophically.
Assembly Biogenesis Photosystem Ⅱ Psb27 Structure Photoinactivation
Peter D Mabbitt Gilles J Rautureau Catherine L Day Mark G Hinds Sigurd M Wilbanks Julian J Eaton-Rye
Department of Biochemistry, University of Otago, Dunedin 9054, New Zealand Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Australia
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
432-435
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）