Comparative Photosynthetic Analyses of Three Widely Used Arabidopsis Ecotypes
There are over 1000 Arabidopsis ecotypes distributed around the world. In this study we have selected three ecotypes which are commonly used for generation of mutant lines, have distinct geographic distribution and plant morphology, namely Columbia Col-0 (Columbia USA), Landsberg erecta Ler-0 (Germany) and Wassilewskija Ws-4 (Belarus). Similar levels of photosynthetic pigment protein complexes were determined in the three ecotypes. Nevertheless, as compared to Col-0 and Ler-0, Ws-4 displayed 50％ lower levels of phosphorylation for the Photosystem Ⅱ D1 and D2 proteins in thylakoids isolated from dark-adapted, growth light- or high-light-treated plants. These results were obtained using western blotting with phosphothreonine antibodies, and confirmed by quantitative mass spectrometry. Furthermore, Photosystem Ⅱ was more susceptible to high light treatment, and the Dl protein was faster degraded in Ws-4 leaves. Our results provide important information for understanding the mechanism underlying natural variation of high light acclimation in Arabidopsis.
Arabidopsis Ecotype High light stress Photosystem Ⅱ Protein phosphorylation Thylakoid membrane
Lan Yin Rikard Fristedt Alexander V Vener Cornelia Spetea
Department of Physics, Chemistry and Biology,Link(o)ping University, Link(o)ping, Sweden Department of Clinical and Experimental Medicine,Link(o)ping University, Link(o)ping, Sweden
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
583-585
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）