Conversion of Chl a into Chl d by Horseradish Peroxidase
We report for the first time on the Chl a into Chl d conversion with horseradish peroxidase in aqueous acetone at room temperature in the dark, both in the presence and absence of hydrogen peroxide, H2O2. After 48 h incubation, the Chl d formation from Chl a catalyzed by horseradish peroxidase was observed in acetone/water (10/1, v/v) even in the absence of H2O2, where the conversion yield, 0.1％, was much lower than that observed in the case of papain, 2％. Very fast conversion occurred with the combination of horseradish peroxidase and dilute H2O2 (acetone/0.3％ H2O2 =10/1, v/v); the yield reached up to 1％ for 2 h incubation. However, the converted Chl d was then gardually degraded probably due to the oxidation power of H2O2. In contrast, such a rapid conversion was not observed in the case of papain, where the conversion yield was, rather, depressed from 2％ down to 0.2％ by H2O2. These results suggest that chemical mechanisms for the Chl a into Chl d conversion catalyzed by papain and horseradish peroxidase are essentially different from each other, which will provide new insight into the unsolved question as to the birth of Chl d in nature.
Acaryochloris marina Chl a Chl d Horseradish peroxidase Hydrogen peroxide(H2O2) Papain
Hayato Furukawa Masami Kobayashi Keisuke Aoki Shingo Itoh Yasuhiro Abe Masataka Nakazato Koji Iwamoto Yoshihiro Shiraiwa Hideaki Miyashita Masaaki Okuda
Institute of Materials Science, University of Tsukuba, Japan Chlorophyll Research Institute, Higashiyachiyo, Yamanashi, Japan Institute of Biological Sciences, University of Tsukuba, Japan Graduate School of Human and Environmental Studies, Kyoto University, Japan
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
799-803
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）