Nonenzymatic Formation of Chl d from Chl a with Hydrogen Peroxide
Nonenzymatic conversion of Chl a to Chl d was performed for the first time by dilute hydrogen peroxide (H2O2) in aqueous acetone (acetone/dilute H2O2 = 10/1, v/v) at 303 K in the dark. The conversion was observed within five minutes incubation when 0.3％ H2O2 was used. The Chl d production yield reached to the maximum, ca. 0.41％, at the incubation time of 5 min. The Chl a to Chl d conversion was also observed when 3％ H2O2 and 0.03％ H2O2 were used, but the maximum yields were much lower, 0.05％ and 0.16％, respectively. The converted Chl d was then gradually degraded probably due to the strong oxidation power of H2O2. Our findings indicate that Chl d is apt to be produced as one of the degradation products of Chl a in nature.
Acaryochloris marina Chl a Chl d Hydrogen peroxide Oxidation Papain
Keisuke Aoki Shingo Itoh Hayato Furukawa Masataka Nakazato Koji Iwamoto Yoshihiro Shiraiwa Hideaki Miyashita Masaaki Okuda Masami Kobayashi
Institute of Materials Science, University of Tsukuba, Japan Chlorophyll Research Institute, Higashiyachiyo, Yamanashi, Japan Institute of Biological Sciences, University of Tsukuba, Japan Graduate School of Human and Environmental Studies, Kyoto University, Japan
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
808-811
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）