Establishing a Core Collection of Pleurotus ostreatus Cultivated in China Based on SSR Markers
To improve the utilization efficiency of Pleurotus ostreatus germplasm, a method for constructing the core collection of P. ostreatus based on molecular marker data was proposed. Using genotype information at 11 SSR makers for 48 P. ostreatus cultivars, an allele preferred sampling strategy was used to construct a P. ostreatus core collection using the UPGMA cluster method according to the genetic distances and the phenotypic traits. The size of each core collection was determined when the ratio of SSR alleles retained was maintained at 100％ , 95％ , 90％ , 85％ and 80％ respectively. The ratio of SSR rare allele retained and the t-test results of Neis gene diversity and Shannons information index were used to evaluate the representativeness of the core collections. This was followed by analyses of the phenotypic representativeness of the core collections, including the coincidence rates of range, mean, and standard deviation between the core collections and the original samples. Our analyses identified that the allele preferred sampling strategy could be used to construct the most representative core collections with the least samples when the ratio of SSR alleles retained was maintained at 95％. In brief, the results indicate the following strategy as a suitable method for constructing P. ostreatus core collection: using allele preferred sampling strategy to sample, determining the size of core collection according to ratio of SSR alleles retained, finally validating representative of the core collection with some phenotypic evaluating parameters.
Pleurotus ostreatus Core Collection Sampling Method Phenotype SSR Genetic Diversity
Chenyang Huang Hui Li Qiang Chen Jinxia Zhang
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
83-91
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）