Genomics of Shiitake Mushroom Lentinula edodes and a Genomic Analytical Platform for Mushrooms
Lentinula edodes ( Shiitake/Xianggu) is a popular cultivated mushroom species. Understanding the genomics of L. edodes is essential to improve its cultivation and quality. Genome sequencing of L edodes provides numerous molecular genetic markers for breeding and genetic manipulation. We sequenced the genome of L. edodes monokaryon L54A using Roche 454 and ABI SOLiD. Sequencing reads of about 1011 Mb were de novo assembled into a 39. 8 Mb genome. We have been annotating the genes and analyzing the metabolic pathways. Over 13000 gene models were predicted from the genome sequence. The gene models were annotated by BLASTX and categorized according to Gene Ontology ( GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Whole genome comparison of L. edodes with several other basdiomycetes and ascomycetes was performed to reveal the phylogenetic relationship. Comparing protein families of the fungal genomes identified protein families conserved to mushroom-forming fungi. We compiled the genome sequences of L. edodes and other fungi into a mushroom genome analysis platform. We are learning more about the molecular biology and genetics of this economically important mushroom.
Lentinula edodes Genome Sequencing Comparative Genomics
Hoi Shan Kwan Qianli Huang Man Kit Cheung Chun Hang Au Man Chun Wong Jing Qin Iris Sze Wai Kwok Winnie Wing Yan Chum Pui Ying Yip Kin Sing Wong Lei Li
School of Life Sciences and Food Research Centre, The Chinese University of Hong Kong, Shatin, Hong Kong, China
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
131-137
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）