Cloning and Quantitative Analysis of Genes Specifically Expressed in Mycelium and Primordium of Pleurotus ostreatus
Digital gene expression profilings (DGE) of two important developmental stages of Pleurotus ostreatus, mycelium and primordium, were constructed using next generation sequencing technology. To clone the specifically expressed genes, we use NlaⅢ digested double-strand cDNA of P. ostreatus as template, unmatched tag as upstream primer, AUAP adaptor primer ( 5-GGCCACGCGTCGACTAGAC-3) as downstream primer for PCR amplification to get corresponding 3cDNA sequence. Totally 120 tags of high, medium, and low expression level in mycelium and primordium of P. ostreatus were selected. After sequencing and blast, we obtain 3 cDNA sequences of 42 genes, among which 18 genes expressed specifically in the mycelium stage and 24 genes are specifically expressed in primordium stage. Full length cDNA sequences of hydrophobin 2 and myosin regulatory light chain genes were obtained by 5 RACE, meanwhile their expression level were confirmed by real-time fluorescence quantitative PCR. This study has laid the foundation for further study molecular mechanism of differentiation and development from mycelium to primordium in P. ostreatus.
Pleurotus ostreatus Specifically Expressed Gene Mycelium Primordium DGE RACE qPCR
Chaomin Yin Yeye Chen Hanyu Zhu Jinghang Lei Aimin Ma
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
161-167
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）