Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola
The methods of degenerate PCR and DNA walking were used to clone a fragment of 4 274bp nucleotides from A. salicacla strain YAASM0711. With sequence alignment and prediction, one mating gene encoding pheromone receptor was found to be 1194bp nucleotides long and included four introns and five extrons with the lengths of 217bp, 113bp, 67bp, 138bp and 449bp respectively. The spliced open reading frame (ORF) contains 984bp nucleotides encoding 327 amino acid residues, and includes seven transmembrane domains, similar to those from Coprinus cinerea and Laccaria bicolor pheromone receptors. The special primers designed flanking the ORF can separate B1 from B2 mating type strains, indicating that they can be used to distinguish mating types among strains.
Agrocybe salicacla Pheromone Receptor Mating Type
Weimin Chen Hongmei Chai Yongchang Zhao
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences Yunnan Provincial Key Lab of Agricultural Biotechnology Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunmi
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
168-174
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）