Development of EST-SSR Markers in Pleurotus ostreatus and Construction of Primary Core Collection
Based on the EST sequences published on NCBI, 9 Pleurotus ostreatus EST-SSR primer pairs, 58 Ganoderma lucidum EST-SSR primer pairs and 35 Lentinula edodes EST-SSR primer pairs were designed. Among these, 4 with good polymorphisms and clear distinctions were selected to analyze 185 strains of Pleurotus ostreatus. After amplification, 59 alleles were generated, 12-19 alleles for each primer, with the fragment sizes between 100 bp and 1 100 bp. A primary core collection, P-core 47, composed of 47 accessions was constructed based on the amplification results. Analysis identified that the P-core 47 accounted for 25 ％ in the original sample pool and 13 ％ in the original resource pool. As for the number of effective alleles and the ratio of polymorphic sites, consistency was preserved between P-core 47 and the original pool. In addition, compared to the original pool, diversity index of Pcore outperformed by 0. 12747, and alleles used to be of low frequency in the original pool were strengthened as well. The strains in P-core 47 are broadly distributed, available in most cultivation regions. Indeed you can find representative alleles associated with both qualitative and quantitative traits in this collection. This study showed the feasibility of adopting EST-SSR markers to construct a primary core strain collection of the Pleurotus ostreatus.
Pleurotus ostreatus EST-SSR Primary Core Collection
Qiang Yao Xue-mei Zhang Zhi-yuan GONG Xing-xi Gao Jin Li Jian-dong Han Peng-fei Ren Lu-zhang Wan Hai-xia Ren
Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jina College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China Institute of Mycological Science and Techonolgy, Ludong University, Yantai 264025, China
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
180-188
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）