Molecular Cloning and Sequence Analysis of DNA Topois-omerase II Gene from Omphalotus nidiformis and Its Application to Phylogenetic Analysis
A fragment of TopoⅡ was amplified from an Omphalotus nidiformis cDNA clone by nested polymerase chain reaction and primer walking. The full-length gene sequence was obtained with 3 and 5-full RACE-PCR. The length of open reading frame is 4 674 bp. The analysis of nucleotide acids sequence exhibits strong sequence homology to other eukaryotic topoisomerase II, and shares 44％ -65％ sequence consistent and 60％ -78％ sequence similarity with the reported TopoII. It shares 65％ sequence identity with TopoII from Pleurotus ostreatus (ABG73430) and 62％ identity with the one from Coprinus cinereus (BAD86854) at the amino acid level. Topo II from P. ostreatus and 0. nidiformis have the same signal sequence with eukaryotic Topo II (LIVMA-X-E-G-DN-S-A-X-STAG) , also have Toprim region and tyrosine activated site.
Omphalotus nidiformis Nested PCR Phylogenetic Analyses
Chenyang Huang Cuixin Li Qiang Chen Jinxia Zhang
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
189-192
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）