Development of Species Level DNA Barcodes to Identify Cultivated Edible Mushrooms
The majority of edible mushrooms belong to Basidiomycetes and these mushrooms have become more important in recent times for their medicinal and nutritional properties. For many years, species of this phylum have been mainly classified by their phenotypic traits, however, taxonomic identification based solely on morphological features can be misleading and unreliable. In contrast, DNA based identification provides a powerful and reliable method for taxonomic discrimination of fungi, it can be performed at any growth stages using parts of the fruit body, mono-and dikaryotic mycelia, or any other organic fungal. In the current study, two different molecular markers including Internal Transcribed Spacer (ITS) I and II and Intergenic Spacer (IGS) I were developed to identify the common cultivated edible mushroom species. Phylogenetic trees could clearly distinguish the analysed species by showing distinct clades. Species differentiations were reconfirmed by AMOVA analysis, nucleotide divergence, haplotyping and Pvalues. Moreover, the designed primers could be employed in phylogenetic studies of other Basidiomycetes. Polymorphism occurred throughout the regions of interest due to insertiondeletion and point mutations, and differentiated the analysed families as well as genera. This study proved that the two developed molecular markers can be used for taxonomic identification of the common cultivated edible mushrooms in Basidiomycetes.
Basidiomycetes ITS IGS DNA Barcode Taxonomic Identification
Farhat Ahmadi Avin Vikineswary Sabaratnam Tan Yee Shin Subha Bhassu
Mushroom Research Centre (MRC) Centre for Biotechnology in Agriculture Research (CEBAR) , Division o Mushroom Research Centre (MRC) Centre for Biotechnology in Agriculture Research (CEBAR) , Division of Genetics and Molecular Biolog
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
255-261
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）