Analysis of Gene Expression Differences in the Substrate-Decomposing Ability Degenerated Strains of Agaricus bisporus
To investigate the gene expression differences and molecular mechanism of substrate-decomposing ability degeneration in Agaricus bisporus (J. E. Lange) Imbach, the strain As2796, cultivated most widely in China, as well as two substrate-decomposing ability degenerated mutants derived from which, 2796-3 and 2796-5, were analyzed by using the techniques of mRNA differential display reverse transcription-PCR ( DDRT-PCR) and two dimensional electrophoresis (2-DE). As a result, five special genes showing obviously differential expressions were found in DDRT-PCR, and some of them have high homologies with substratedecomposing enzymes. Based on the genome sequence data of A. bisporus, PCR primers were designed, and the coding sequences (CDS) with introns and about 1200 bp upstream regulative sequences of above five special genes were amplified, cloned and sequenced, but the comparison of the sequences among three strains showed that the genes and upstream sequences have no repeatable difference. In the 2-DE analysis, two proteins were found to be up-and down-regulated expression, respectively, with obvious and repeatable profiles in both degenerated strains. Based on the results of MALDI-TOF/MS analysis and database searching, these two proteins were identified as an actin and a putative NADH dehydrogenase iron-sulfur protein 3, respectively.
Agaricus bisporus As2796 DDRT-PCR 2-DE Gene Cloning
Meiyuan Chen Jianhua Liao Zhixin Cai Hongrong Li Zhongjie Guo Zesheng Wang
Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, China
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
284-292
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）