Characterization of Mating-Type Genes,Determination of Nuclear Phase and Strain Identification in Rhizopogon roseolus
In the current studies, we sequenced and characterized the genes for homeodomain protein (RrA1-hox1, RrA1-hox2) and its flanking region mip gene in a bipolar ectomycorrhizal basidiomycete, Rhizopogon roseolus. Linkage analysis indicated that the sequenced region is mapped to A mating type locus in R. roseolus. By nucleotide alignment analysis of partial nucleotide sequences of two Rrhox1 genes from two mating compatible monokaryotic strains, we deduced 3-region of Rrhox1 gene sequences in R. roseolus has a divergence-homogenization duality, which makes the restriction fragment length polymorphism ( RFLP) analysis of this region possible. Using the primer pair designed according to 3-region of RrAl-hox1 and mip gene in A1 mating type, all ten dikaryotic strains of R. roseolus collected from nine prefectures of Japan were got the target PCR amplification products. After digesting the target PCR products by two restriction enzymes, AluI and RsaI, respectively, both RFLP patterns showed diversity and ten R. roseolus strains were well distinguished. So we concluded that 3-region of homeodomain protein gene ( Rrhoxl ) and mip gene is suitable for strain identification by RFLP analysis in R. roseolus.
Homeodomain Mating Type RFLP ( restriction fragment length polymorphism ) Pheromone Receptor
Jianing Wan Yan Li Norihiro Shimomura Takeshi Yamaguchi Tadanori Aimi
The United Graduate School of Agricultural Sciences, 4-101 Koyamacho Minami, Tottori 680-8553, Japan Faculty of griculture, Tottori University, 4-101 Koyamacho Minami, Tottori 680-8553, Japan Faculty of Agriculture, Tottori University, 4-101 Koyamacho Minami, Tottori 680-8553, Japan
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
315-325
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）