Detection of Trichoderma aggressivum Green Mould During Spawn-run
Since 2006, green mould ( Trichoderma aggressivum) has presented big problems to the Dutch mushroom industry. T. aggressivum infects compost at a very early stage and in the Dutch situation infection most likely takes place at the compost yard. Even though compost producers in the Netherlands are very keen to prevent green mould problems, occasionally still a number of crops get infected. Therefore there is a need for a method that allows early detection of Trichoderma green mould. Agaricus bisporus and T. aggressivum use volatiles to affect each others growth rate. We tested the possibility of detecting Trichoderma green mould using the volatiles that are produced during spawn run. We used an untargeted metabolomics approach to compare the volatiles emitted by non-infected compost with those of infected compost. A proof of principle for this approach was obtained in an experimental model containing 300 g of freshly spawned, well aerated phase 2 compost. This model compared a development into fully infected compost with a normal spawn-run. In commercial practice of phase 3 composting, tunnels are likely partially infected. We therefore tested whether the method would also work in 50 kg batches of partially infected compost. The potential of using headspace volatiles asa detection method to detect T. aggressivumin the process air of the tunnels, avoiding the need to sample inside the tunnel is discussed.
Agaricus bisporus Trichoderma aggressivum Green Mould Volatiles Compost Headspace Analysis Gas-chromatography Mass Spectrometry Metabolomics
Johan Baars Jo Rutjens Roland Mumm
Plant Research International, P.O. Box 16, Wageningen 6700AA, The Netherlands Plant Research International, P.O. Box 16, Wageningen 6700AA, The Netherlands Centre for BioSystems
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
343-349
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）