Anticancer Potential of Mushroom Extracts from Commercially Cultivated Species in La Rioja, Spain
The World Health Organization ( WHO) considers that, giving the current trends, cancer will be the first cause of mortality in the world within a few years. A great deal of economic and scientific effort is devoted annually to finding new cures for this disease, but most of the current therapies produce severe side effects. In this study, we have tested the antiproliferative properties of several species of cultivated mushrooms that are produced in bulk quantities in commercial exploitations of La Rioja, Spain. Mushrooms were freeze-dried, grinded to powder form, and dissolved in culture medium at the proper concentrations. The lung cancer cell line A549 was used as a model. These cells were seeded in 96-well plates and exposed to increasing concentrations of mushroom extracts for 5 days in the absence of fetal bovine serum. At the end of the experiment, cell number was estimated with Promegas Cell Titer solution and quantified in a plate reader. Five varieties were studied: Agaricus bisporus, Agaricus bisporus grown in a selenium-enriched substrate, Pleurotus ostreatus, Pholiota aegerita, and Lentinus edodes. All mushroom extracts produced a dose dependent reduction in cancer cell growth, although the calculated IC50 was different depending on the species and the variety. Agaricus bisporus was the less efficient species (IC50 = 4.0 mg/mL) , although the selenium-enriched mushrooms were a little more efficient (IC50 =3.0 mg/mL). Pleurotus ostreatus (IC50 = 0. 10 mg/mL) and Pholiota aegerita ( IC50 = 0. 15 mg/mL) induced more cell growth arrest, and Lentinus edodes ( IC50 = 0.06 mg/mL) was the most efficient species in reducing cancer growth. Further studies are under way in our laboratories to better understand which parts of the mushrooms and stages of the mushroom cycle contain higher concentrations of anticancer agents.
Agaricus bisporus Pleurotus ostreatus Pholiota aegerita Lentinus edodes Lung Cancer
Marcos Vilari(n)o Cristina Clavijo Margarita Pérez Alfredo Martínez
Oncology Area, Center for Biomedical Research of La Rioja ( CIBIR) , Logroiio, La Rioja, Spain Technological Center for the Study of Mushrooms (CTICH), Autol, La Rioja, Spain
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
483-490
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）