Antibacterial Potential of Lenzites betulinus Cultivation Medium Enriched with Selenium
Lenzites betulinus (L. ) Fr. is one of the most famous medicinal mushroom species which has been used in traditional Chinese medicine for centuries. Current scientific studies have confirmed wide range of its therapeutic effects, as immunomodulating, antioxidant and antimicrobial activity. Selenium is one of the trace elements of fundamental importance to human health, therefore the aim of this study was to examine antibacterial activity of the cultivation medium enriched with selenium in defined initial concentrations after 21 days of L. betulinus cultivation. The initial selenium concentrations were 300 μg/L, 700μg/L, 1000 μg/L and 1300μg/L, which decreased after sterilization to the values of 96 μg/L, 210 μg/L, 296 μg/L and 373 μg/L, respectively. The medium without selenium was used as the control. The mycelium was separated from cultivation medium by Whatman No. 4 filter paper and the filtrate was sterilized by Millipore membrane filter (0.2 μm). The selenium concentrations in the filtrates were 6. 1 μg/L, 11.2 μg/L, 14.9 μg/L and 22.4 μg/L, respectivelly. The antibacterial activity was estimated by determination of the minimal inhibitory concentration (MIC) by the serial filtrates dillutions (from 100％ to 0. 195％) on microtiter plates. Three Gram (-) ( Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa) and two Gram ( + ) bacterial species (Staphilococcus aureus and Enterococcus faecalis) were tested. The selenium-enriched filtrates showed antibacterial activity against all tested bacterial species. However, growth of P. aeruginosa was inhibited equally by 50％ filtrates of the control and those with all selenium concentrations. The maximum antibacterial activity ( MIC - 6. 25％ ) was noted for the filtrate with selenium in the concentration of 11. 2 μg/ L against S. aureus. This concentration has also the best antibacterial activity for tested bacterial species. Generally, it could be concluded that selenium added in the lower concentrations (300 μg/L and 700 μg/L) has strong antibacterial activity, especially against Gram ( + ) species.
Antibacterial Activity Cultivation Filtrate Lenzites betulinus Selenium
Milovanovi(c)lvan Kosani(c)Marijana Cilerd(z)i(c)Jasmina Br(c)eski Illja Kne(z)evicAleksandar Staji(c)Mirjana Vukojevi(c)Jelena
University of Belgrade, Faculty of Biology, Serbia, Takovska 43, Belgrade 11000, Serbia University of Kragujevac, Faculty of Science, Radoja Domanovica 12, Kragujevac 34000, Serbia University of Belgrade,Faculty of Chemistry, Studentski Trg 3 , 11000 Belgrade, Serbia
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
491-494
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）