Genomics Changes Induced by Maitake D-Fraction in the Suppression of Breast Cancer Phenotype
It s already known that Maitake (D-Fraction) mushroom is involved in stimulating the immune system and activating certain cells and proteins that attack cancer cells, including macrophages, T-cells, natural killer cells, and interleukin-1 and -2. For centuries, Japanese people have used Maitake mushrooms as a general promoter of robust health. According to the National Cancer Institute, polysaccharide complexes present in Maitake mushrooms appear to have significant anti-cancer activity. Also, it has been suggested that Maitake may work in conjunction with chemotherapy to lessen its side effects and to boost its positive effects. However, the exact molecular mechanism unchage by Maitake in the struggle against the cancerous cell is still unclear. At the present work we are trying to clarify the genomic expression induced by Maitake D-Fraction in MCF-7 breast cancer cells. We also want to demonstrate which genes are involved in the suppression of the breast tumoral phenotype and which biological pathways are involving in this effect. Therefore, we performed a new set of human cDNA microarrays assays including 25000 genes employing MCF-7 cells treated with and without increased doses of Maitake D-Fraction (91, 183 and 367 μg/mL), during 24 hours. The gene expressions were corroborated by real time RTPCR assay employing commercial available primers from Applied Biosystems, INC, CA. Understanding the molecular mechanisms induced by Maitake D-Fraction in the suppression of breast cancer phenotype may lead to the development of therapeutic/preventive strategies against highly incident of breast cancer.
Maitake D-Fraction Breast Cancer MCF-7 Tumoral Phenotype
Alonso Eliana Gabriela Balogh
Biotechnology Laboratory, Centro Cientffico Tecnológico, CERZOS-CONICET, Bahia Blanca, Buenos ires,A Biotechnology Laboratory, Centro Cientffico Tecnológico, CERZOS-CONICET, Bahia Blanca, Buenos Aires,
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
495-501
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）