Lignosus rhinocerus (Cooke) Ryvarden-A New Potential Medicinal Mushroom that Can Stimulate Neurite Outgrowth at Low Concentrations of Extracts
Alzheimers disease is characterized by the loss of neurons and synapses in the cerebral cortex and some subcortical regions. Research has shown that absence of nerve growth factor causes Alzheimer-like symptoms in the adult brain of mice. A well known medicinal mushroom, Hericium erinaceus was found to have active compound/s with NGF-like properties and able to cross blood-brain barrier. The aim of this study was to investigate a new potential mushroom that had been used for medicinal purposes by local community in Malaysia. Neurite outgrowth in cultured rat pheochromocytoma cells, PC-12 acts as an indication of regenerative potential. Differentiated cells with one thin extension at least double the length of the cell diameter were scored positive. Our results showed that maximum outgrowth was shown at 20 μg/mL (w/v) for both L. rhinocerus aqueous and ethanol extract whereas for H. erinaceus maximal outgrowth was shown at 50 (μg/mL (w/v) of aqueous extract and 70 μg/mL (w/v) of ethanol extract. Lignosus rhinocerus extracts showed maximal neurite outgrowth at lower concentrations compared to H. erinaceus extracts. This showed that L. rhinocerus extracts may contain compound/s that able to enhance neurite outgrowth activity at a lower concentration. MTT assay was performed to check the percentage of cell viability after treatment with L. rhinocerus extract. IC50 value of aqueous extract of L. rhinocerus was recorded at 445. 15 μg/mL (w/v) and R value was 0. 988 4 while IC50 value of ethanol extract of L. rhinocerus was 282. 15 μg/mL (w/v) and R value was 0.991 8. IC50 values showed that extracts tested had no cytotoxic effect at low concentrations. To our knowledge, limited work has been done on neurite outgrowth activity using L. rhinocerus. Based on this preliminary result, further development and investigations are needed to confirm the mechanism / pathway for the neurite outgrowth. To our knowledge, this is the first report on neurite outgrowth stimulation activity by Lignosus rhinocerus.
Lignosus rhinocerus Hericium erinaceus Neurite Outgrowth Aqueous Extract Ethanol Extract Cytotoxic
Lee-Fang Eik Murali Naidu Vikineswary Sabaratnam
Mushroom Research Centre, University of Malaya, Kuala Lumpur 50603, Malaysia Institute of Biological Mushroom Research Centre, University of Malaya, Kuala Lumpur 50603, Malaysia Department of Anatomy,
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
630-634
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）