Cereal Grains and Cryoprotectants in Lentinula edodes Cryopreservation at-70C
The periodical transfer of mycelium to cultivation medium is still the most used method in filamentous fungus preservation, but it implies in increasing the risk of contamination and/or genetic degeneration. Other short-term preservation techniques as mineral oil and distilled water have been used for basidiomycetes but they presented variation of morphology, physiology and mycelial viability directly proportional to preservation duration. Cryopreservation is considered the safest and most reliable and effective method to preserve fungi due to metabolism inactivation, and it is the one with the lowest contamination and genetic degeneration risk, mainly for basidiomycetes. However,because of the diversity of fungus species and the different responses to cryopreservation process,alternatives are necessary for each species cryopreservation,mainly among basidiomycetes. The objective of this study is to evaluate substrates associated to cryoprotective agents for Lentinula edodes cryopreservation at -20℃ and at -70℃. Mycelia were grown on oat, wheat, rice or millet grains or malt extract, immersed in cryoprotectants such as dimethyl sulfoxide,glycerol,saccharose,glucose,polyethylene glycol-6000 or malt extract, and cryopreserved at - 20℃ or at - 70℃. After one-, two-or three-year cryopreservation mycelial viability was verified. Cryopreservation at -20℃ is ineffective. For threeyear cryopreservation at -70℃ wheat grains are the most consistent for mycelial viability among treatments and saccharose is the best cryoprotectant with mycelial viability of 87％. Cryopreservation at 70℃ is effective after three years,but not at -20℃ even after one year. Wheat grains and saccharose are the most effective protocol for mycelial viability in L edodes cryopreservation at -70℃ for three years.
Lentinula edodes Cryopreservation Substrate Cryoprotectant
Talita Rafaele DAgostini Mantovani Henrique Susumu Tanaka Suzana Harue Umeo Lienine Luiz Zaghi Junior Luzia Doretto Paccola - Meirelles Giani Andrea Linde Nelson Barros Colauto
Molecular Biology Laboratory, Postgraduate Program in Biotechnology Applied to Agriculture, The Para Biological Sciences Center, State University of Londrina, Parana,Londrina, Brazil
国际会议
The 18th Congress of the International Society for Mushroom Science(第十八届国际食用菌大会 ISMS18)
北京
英文
887-894
2012-08-27（万方平台首次上网日期，不代表论文的发表时间）