Fabrication and Application of the Nano-Biosensor based on the Liposome for Detection of Pesticides
Monitoring of the organophosphorus pesticides dichlorvos at very low leveb has been achieved with liposome-based nano biosensors. The enzyme acetylcholinesterase was effectively stabilized within the internal nano-environment of the liposomes. Within the liposomes, the pH sensitive fluorescent indicator pyranine was also immobilized for the optical transduction of the enzymatic activity. Increasing amounts of pesticides lead to the decrease of the enzymatic activity for the hydrolysis of the acetylcholine and thus to a decrease in the fluorescent signal of the pH indicator. The decrease of the liposome biosensors signal is relative to the concentration of dichlorvos down to 1012 M levels. Abo a colorimetric screening device for pesticide analysis has been evaluated.
Biosensor Liposome Pesticides Acetylcholinesterase
Hua-nan Guan
Key Laboratory for Food Science and Engineering Harbin University of Commerce Harbin, China
国际会议
哈尔滨
英文
143-146
2012-06-16（万方平台首次上网日期，不代表论文的发表时间）