Fingerprint analysis of Polygonatum odoratum (Mill.) Druce using high-performance liquid chromatographic method with diode-array detection
The fingerprints analysis of different lines of Polygonatum odoratum (Mill.) Druce were established by high-performance liquid chromatographic with diode-array detection (HPLC/DAD). The analyses were performed on ZORBAX Eclipse XDB-C18 (4.6×50 mm, 5 μm) column using a mobile phase (aqueous 0.05% formic acid-acetonitrile) under gradient conditions. The flow rate was 1.0 ml/min. The detecting wavelength was set at 210 nm. HPLC fingerprints of two lines were little different. The similarity of the same line was greater than 0.9, and the average similarity of the different lines was 0.794. This method could be suitable for the line identification and quality control of Polygonatum odoratum (Mill.) Druce.
Polygonatum odoratum (Mill) Druce high-performance liquid chromatographic method Fingerprint
Guangsheng Xi
Department of Chinese Traditional Medicine Science Jilin Agriculture Science and Technology College Jilin, China
国际会议
哈尔滨
英文
204-206
2012-06-16(万方平台首次上网日期,不代表论文的发表时间)