Molecular Cloning and Characterization of PDK1 gene from Inner Mongolia Cashmere Goat (Capra hircus)

摘要：

PDK1, 3-phosphoinositide-dependent protein kinase-1, is a member of the AGC family. It contributes in regulating many processes including cell metabolism, growth, proliferation, and survival. In this study, PDK1 partial cDNA was amplified by RT-PCR from Inner Mongolia Cashmere goat (Capra hircus) and sequenced. The molecular characterization of the PDK1 gene was described. The amplified fragment is 1141bp in length and CDS is 1008bp, corresponding to a polypeptide of 335 amino acids residues with 3831 kDa predicted molecular mass. The cDNA nucleotide sequence has 88％97％ identity with that of human and bovine. The goat PDK1 partial cDNA was cloned.

关键词： Cashmere goat PDK1 gene clone

作者: Y. H. Chen W. N. Qing Y. F. Wang H. X. Fu Z. G. Wang

作者单位: College of Life Science, Inner Mongolia University,The Key Laboratory of Mammal Reproductive Biology and Biotechnology,Hohhot 010021, China

会议类型: 国际会议

会议名称: The 2012 International Conference of Agricultural Engineering and Food Engineering(2012年国际农业工程与食品工程学术会议 ICAE 2012)

会议地点: 哈尔滨

会议语种:英文

页码: 262-265

在线出版日期: 2012-06-16（万方平台首次上网日期，不代表论文的发表时间）