Assessment of Biological and Biochemical Indicators in Saline-alkali Soil under Transgenic BADH and non-BADH Soybean Crop in Northeast China
The gene encoding for betaine aldehyde dehydrogenase (BADH) plays an important part in plant molecular breeding for stress resistance. The transgenic crops carrying BADH gene enhance tolerance to saline-alkali and drought stresses. But there is concern that transgenic BADH-crops could have undesirable effects on natural and agro-ecosystem functions. We investigated the effect of soybean carrying BADH gene on several microbial and biochemical indicators in a saline-alkali agro-ecosystem. BADH-soybtun (SRTS) and its non-transgenic near-isoline (HN35) were grown on a soda-saline soil. Soil and root samples were collected 30,60, 90, 120, 150 days after sowing (DAS). Soil from a control (no-crop) treatment was also included. Samples were analysed for microbial biomass C, N and P (MBC, MBN, MBP), total organic carbon (TOC), and several soil enzyme activities. The microbial quotient (MQ) was calculated as the ratio of MBC-to-TOC. The average of the five sampling events revealed a significant increase in MBC, MBN, and MQ in the soil under BADH-soybean over the nonBADH isoline. On the contrary, non-BADH soybean had higher MPB than BADH soybean. The TOC was similar in BADH and non-BADH systems. Soil under BADH-soybean had higher potential N mineralization, nitrification, nitrate reductase and acid phosphatase activities, and lower alkaline phosphatase activity than its under non-BADH soybean. Root length, root volume and root biomass weight did not vary (P > 0.05) between BADH and non-BADH soybean at 30 DAS, but there were significant differences with a greater root parameters with BADH-soybean thereafter. The time of sampling strongly affected the above parameters, with most being highest on 90 DAS. We concluded from the data that there were some positive or no negative effects of BADH-soybean on the studied indicators, and therefore cultivation of BADH-soybean appears to be no risk to saline-alkali soil ecosystem functions.
saline-alkali soil transgenic BADH-soybean microbial biomass soil enzymes assessment
Yu Song Liu Zhihua Zhou Jie Shao Jingxin Zhang Jue Wang Hongyan
Resourcas and Environment Sciences College Northeast Agricultural University Harbin, China Resources and Environment Sciences College Northeast Agricultural University Harbin, China
国际会议
哈尔滨
英文
416-422
2012-06-16（万方平台首次上网日期，不代表论文的发表时间）