Selection and Evaluation of Potential Probiotic Lactic Acid Bacteria and Bifidobacteria Isolated from Human Intestinal Tract Selection and evaluation of potential probiotic
Three Lactobacillus strains were screened on the basis of probiotic characteristics (i.e., resistance to low pH and bile salts, adhesion to the human gastrointestinal tract, inhibition of pathogenic strains). They further exhibited producing antimicrobial activities of non-acid molecule(s). In addition, antibacterial peptides were isolated and purified from the cell-free culture supernatants of these three probiotic strains. Based on Tricine-SDSPAGE, the antimicrobial peptide was approximately 10 kDa in size. After analyzing the sequence of the 16SrDNA regions of these three strains, they were identified as Lactobacillus crispatus Lactobacillus rhamnosus and Lactobacillus rhamnosua GG. Using an in vitro system simulating gastric transit, our findings indicated that the three probiotic strains had the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells. It was demonstrated that the probiotic strains inhibited subsequent adhesion of E. coli to the HT-29 cell. Among the selected strains, L. rhamnosus F1333 showed a high probiotic potential and could be used in healthpromoting food products.
Probiotics Transit tolerance Antibacterial activity Antibacterial peptides
Chaohui Xue Xue Han Lanwei Zhang Hongbo Li Shumei Wang Chunfeng Guo QiLi Xue Luo Ming Du Huaxi Yi
School of Food Science & Technology Harbin Institute of Technology Harbin, China
国际会议
哈尔滨
英文
623-626
2012-06-16（万方平台首次上网日期，不代表论文的发表时间）