Application of High-Speed Counter-Current Chromatography for Isolation of Triterpenes from Schisandra Chinensis(Turcz.) Baill and Induction Apoptosis Mechanism of HSC-T6
Following an initial cleaning-up step on the AB-8 macroporous resin, a preparative high-speed countercurrent chromatography (HSCCC) with a two-phase solvent system composed of Chloroform-n-Butyl alcohol- methanol-water (10:1:7:4, v/v) was used to isolate and separate triterpenes from caculis of Schisandra Chinensis(Turcz) baill. A total of 89 mg corosolic acid with purities of 98.5％ were obtained from 400 mg crude extract in one-step elution and less than 4 h, and the structure identification was performed by UV, IR, MS, 1H NMR and 13C NMR. The inhibiting on liver fibrosis activities of the triterpenes against HSC-T6 in vitro were studied by cell culture methods. The results showed that the corosolic acid had better inhibitory effects against HSC-T6 cells with the IC50 value of 5～25 V g/Ml., and the study also indicated that corosolic acid might be a potential Chinese medical component for inhibiting liver fibrosis.
Schisandra Chinensis(Turcz.) baill Triterpenes liver fibrosis
Bin Li Xianjun Meng Lijie Zhu Xv Si
College of Food Science Shenyang Agricultural University,Shenyang, China
国际会议
哈尔滨
英文
668-672
2012-06-16（万方平台首次上网日期，不代表论文的发表时间）