Expression of Soluble Recombinant Transglutaminase from Zea mays in Pichia Pastoris
Transglutaminases (TGases) are a family of enzymes that catalyse the cross-linking of proteins and widely used in the food industry to improve the texture of dairy, meat, bread products. Zea mays TGase (ZTGase) is a new type of TGase with wide developing prospect. To further research the application and function of this enzyme, ZTGase gene was introduced into Pichia pastoris expression vector pPIC9K and the resultant plasm id pPIC9K-TGZ was transformed into the P. pastoris GS115 by electroporation. The products of the positive transformants induced by methanol were analyzed by SDS-PAGE. The results showed that the ZTGase gene was successfully inserted into pPIC9K vector and ZTGase was expressed in P. pastoris. The size of induced product was identical to the calculated molecular mass, and the activity of recombinant ZTGase was 0.321 U/mg protein. The results indicated that this is an efficient method to obtain active, soluble ZTGase protein.
Transglutaminase Zea mays Pichia pastoris GS115 Protein expression
Hongbo Li Lanwei Zhang Yanhua Cui Chaohui Xue Shumei Wang Xue Luo
School of Food Science & Technology Harbin Institute of Technology Harbin, China
国际会议
哈尔滨
英文
742-745
2012-06-16（万方平台首次上网日期，不代表论文的发表时间）