Development and Experimental Research of the Bioreactor Using Taraxacum Kok-saghyz Laticifers
Taraxacum koksaghyz Rodin (Russian dandelion) is one of the most important major rubber -producing plants in the world. Its laticifers can be act as bioreactor to study the overexpression or down regulation of key genes that are involved in rubber biosynthesis. As a model rubber plant, the experiment can be designed in two steps. One step is to construct the tissue culture system of T. koksaghyz, the other step is to construct the genetic transformation system and then transform plant expression vector into the T. koksaghyz. In this paper, plant expression vector pBI121-T14 containing the 35S promoter was selected to transfer into T. koksaghyz. The results showed that the tissue culture and the genetic transformation system of T. Koksaghyz have been successfully established, and the pBI121-T14 containing the 35S promoter was transformed into T. Koksaghyz by leaf disc transformation method. The expression product of GUS gene has been detected in transgenic T. Koksaghyz. In the following research, the plant expression vector (including Taxol synthase gene) with latex-specific promoter will be transferred into the T. Koksaghyz to further study about the mechanism of bioreactor.
laticifer bioreactor Taraxacum Koksaghyz tgenetic transfonrt rans-gene
Suyu Liang Lin Li WenfanWang TienanLiu QianDu Guangju Liu Songyan Tian
Forest Engineering and Environment Research Institute of Heilongjiang Province Harbin, China
国际会议
哈尔滨
英文
763-765
2012-06-16（万方平台首次上网日期，不代表论文的发表时间）