Expression and Purification of Tomato Cnr (colorless non-ripening) Protein in Escherichia Coli

摘要：

Tomato fruit ripening mutant colorless non-ripening (Cnr) is an autosomal dominant mutant, obtained by natural mutation. The fruit of Cnr mutant was obviously immature, and the softening and lycopene accumulation of the Cnr fruit was significantly inhibited during the fruit ripening. In this work, we cloned the Cnr gene from the red ripe tomato fruit by RT-PCR, and constructed a prokaryotic expression vector. And the recombinant Cnr protein was induced by 1 mM IPTG in Escherichia coli BL21 (DE3), and purified by MBP affinity chromatography.

关键词： tomato colorless non-ripening prokaryotic expression affinity chromatography

作者: Yuanhong Xie Hui Liu Hongxing Zhang

作者单位: Beijing Key Laboratory for Agricultural Application and New Technique Beijing, China College of Food Science Beijing University of Agriculture Beijing China

会议类型: 国际会议

会议名称: The 2012 International Conference of Agricultural Engineering and Food Engineering(2012年国际农业工程与食品工程学术会议 ICAE 2012)

会议地点: 哈尔滨

会议语种:英文

页码: 864-866

在线出版日期: 2012-06-16（万方平台首次上网日期，不代表论文的发表时间）