Comparing Effect of Extraction Methods on Polysaccharide of Clitocybe maxima Stipe
Clitocybe maxima (Gartn. ex Mey.: Fr.) Quel. is a favorable edible mushroom. As a bio-waste (or defined as byproduct), it produces a high proportion of biomass in mushroom stipe for about 45％ of entire fruits. To increase its value added utilization, three extraction methods (as hot water, microwaveassisted and complex-enzyme-hydro lysis-assist) were conducted. The extraction effect on the polysaccharide of Clitocybe maxima stipe was compared and the processing conditions in extraction were optimized. The content of Clitocybe maxima polysaccharide was determined with Phenol Sulfuric Acid Method. The yield and purity of the stipe polysaccharide obtained with three extractions were greatly different, which were 5.86％ and 40.23％ for hot water extraction, 9.4％ and 52.62％ for microwareassist extraction, 10.26％ and 53.13％ for complexenzyme-hydrolysis extraction, respectively. Results indicated that the complex-enzyme-hydrolysis-assist extraction was the best approach for extracting the polysaccharide from Clitocybe maxima stipe with the advantages in high extraction efficiency, energy saving and running time saving.
stipe of Clitocybe maxima yield of polysaccharide extraction methods
Junchen Chen Pufii Lai Hengsheng Shen Hengguang Zhen Rutao Fang
Research Institute of Agri-engneering Technology Fujian Academy of Agricultural Sciences Fuzhou, Fujian, PR China
国际会议
哈尔滨
英文
973-976
2012-06-16（万方平台首次上网日期，不代表论文的发表时间）