Studies on the Isolation, Purification of Cellulase Produced by Aspergillus Niger Ⅲ and Trichoderca Viride and their Enzymatic Properties
Isolation and purification of the crude cellulase produced by apsergillus niger Ⅲ and trichoderma viride Ⅰ, and the enzymatic properties were studied in the paper. The results: By means of ammonium sulfate salting-out methods and sephadex G-100 column chromatography, five elution peaks were obtained, namely peak 1～5. The peak 2 included endoglucanase and exo-glucanase. Peak3 included endoglucanase, exoglucanase, and B-glycosidase. Peakl, 4 were not active, peak 5 included endoglucanase and Bglycosidase. By DEAE FF weak anion exchange chromatography, a kind of endoglucanase was purified from peak 2, and its molecular weight was 61.5kO by SDS-PAGE cataphoresis. A range of pH 5.0～6.0, the CMCase activity of peak 2 maintained 70％～80％ of initial enzymatic activity, and the optimum pH was 5.0;higher enzyme activity appeared at 30～50℃, the optimal temperature was 50℃, and the enzyme activity rapidly decreased when temperature was higher than 60℃.
apsergillus niger trichoderma viride mixed fermentation cellulase isolation and purification
Hongping Hou Sheng Yang
College of Food Science and Engineering Shanxi Agricultural University Shanxi China College of Food Science and Engineering Shanxi Agricultural University The Fruit Trees Science Insti
国际会议
哈尔滨
英文
1000-1003
2012-06-16（万方平台首次上网日期，不代表论文的发表时间）