Effects of Different Neutralizing Agents on D-(-)-Lactic Acid Fermentation by Escherichia coli W

摘要：

Effects of NH4OH,KOH,Ca(OH)2,CaCO3,and a mixture of CaCO3 and NH4OH as neutralizing agents for efficient recovery of lactic acid fermented by Escherichia coli W were investigated.Maximum productivities of lactic acid were 11.3 g/L,10.7 g/L,5.5 g/L,11.9 g/L,and 12.8 g/L respectively.The mixed neutralizing agent of CaCO3 and NH4OH was testified to be better than any single neutralizer used in this study.CaCO3 was the most appropriate neutralizer among the first four neutralizes,which could make lactic acid precipitate in the form of calcium lactate,and facilitate the downstream separation and extraction of lactic acid.Furthermore,it was a slow type of mild neutralizer and had a limited capacity to regulate pH.NH4OH could regulate pH easily,and it was beneficial to cell growth as an additional nitrogen source,thus resulting in a relatively lower lactic acid production.

关键词： D-(-)-lactic acid Escherichia coli W neutralizing agent fermentation

作者: JinfangZhao Kunpeng Li Yongze Wang Jinhua Wang Shengde Zhou

作者单位: Key Laboratory of Fermentation Engineering (Ministry of Education),College of Bioengineering,Hubei U Department of Biological Sciences,Northern Illinois University,DeKalb,IL60115,USA

会议类型: 国际会议

会议名称: 2012 2nd International Conference on Advanced Material Research(2012 第二届先进材料研究国际会议 ICAMR2012)

会议地点: 成都

会议语种:英文

页码: 845-849

在线出版日期: 2012-01-07（万方平台首次上网日期，不代表论文的发表时间）