Development of a Polyclonal Antibody Based Strip Assay for Detection of Cadmium Residue
A rapid immunochromatographic lateral flow test strip has been developed using a gold-conjugated polyclonal antibody (pAb) for the specific determination of Cadmium (Cd2+) residue in environmental water. For this purpose, the isothiocyanate method was employed to synthesize Cd2+ artificial antigen and New Zealand white rabbits were immunized to produce anti-Cd2+ pAb. Antigen and goat anti-rabbit IgG were respectively drawn on NC membrane as test line and control line. Gold-labeled antibody was added on a pad and put on one end of the membrane. The detection limit of this strip was measured to be SO ng/mL. Except for high cross-reactivity (83％) toward Hg2+, no significant cross-reactivity was observed for other metal chelates tested. The results demonstrate that the developed method can be potentially used as a screening tool for the determination of Cd2* residues in a large amount of samples on site.
cadmium artificial antigen polyclonal antibody strip assay environmental water
Wu Shixiu Jiang Jinqing
College of Animal Science, Henan Institute of Science and Technology, Xinxiang 453003, China
国际会议
哈尔滨
英文
1037-1040
2011-12-24（万方平台首次上网日期，不代表论文的发表时间）