THE COMPLEMENTARY DNA SEGMENT CLONING AND BIOINFORMATICS ANALYSIS OF INTS2 GENE IN GOOSE

摘要：

To clone the INTS2 (Integrator complex subunit 2) cDNA segment in goose, we isolated total RNA from goose liver.The 488bp cDNA segment was amplified by the reverse transcription polymerase chain reaction (RT-PCR).The obtained segment had 3 open reading frames (ORF) and two of them are separately 100％ and 99％ relative to the sequences of chicken.One of the segment contains 113 amino acids, 63.7％ of them form helix and 36.3％ loop.And the other 45AA segment contains 22.2％ helix, 17.8％ strand and 60％ loop. Comparison analysis indicated that the nucleotide sequence and amino acid sequence of INTS2 gene segment were highly homologous to some species reported, such as Gallus gallus, Taeniopygia guttata, Ornithorhynchus anatinus ect.Homology between Sichuan White Goose and Gallus gallus was 94％, and Taeniopygia guttata 88％.We confirmed the sequence to be segment of INTS2 gene.

关键词： Sichuan White Goose INTS2 Bioinformatics Analysis

作者: SHUANG MA CHUNCHUN HAN JIWEN WANG

作者单位: Institute of Animal breeding & Genetic,Sichuan Agricultural University

会议类型: 国际会议

会议名称: 2011 3rd International Conference on Computer Technology and Development(2011第三届计算机技术与发展国际会议 ICCTD2011)

会议地点: 成都

会议语种:英文

页码: 822-826

在线出版日期: 2011-11-25（万方平台首次上网日期，不代表论文的发表时间）