Study of non-viral gene transfer of NB4 cells

摘要：

Post-Transcriptional Gene Silencing and RNA interference (RNAi) are terms describing the specific suppression of genes by complementary dsRNA.Highefficiency transfection is an essential first step for achieving effective gene knockdown.Non-viral transfection include chemical-transfection and electrotransfection.For a particular cell line, select the appropriate transfection method is very important.In this study, we compared a variety of commercially available reagents and electrotransfection, approaches to define methods for efficient delivery of siRNA to NB4 cells. Fluorescently-labelled siRNA were tested.We report that different methods have different effects.The results provide guidelines for optimal siRNA-mediated knockdown in the NB4 cells.

关键词： NB4 cells RNAi transfection

作者: Chen Ying Wang Huaiyu Zhang Mei

作者单位: Department of Hematology,the First Clinical Hospital of Xian Jiaotong University,No.1 Jiankang Road,Xian,Shaanxi 710061,China

会议类型: 国际会议

会议名称: 2011 2nd International Conference on Material and Manufacturing Technology(2011第二届材料与制造技术国际会议 ICMMT2011)

会议地点: 厦门

会议语种:英文

页码: 333-337

在线出版日期: 2011-07-08（万方平台首次上网日期，不代表论文的发表时间）