Research of Immobilization of Transglutaminase on Polypropylene Microporous Membranes

摘要：

Activated ozone as the carrier, crylic acid as the monomer and glutaraldehyde as the cross-linking agent were used to research the immobilization of transglutaminase on polypropylene microporous membranes.The effect of ozone activation time, grafting time, grafting temperature, monomer concentration and ammonium ferrous sulfate concentration on grafting degree were studied, the Immobilization conditions were also studied.We found the optimal reaction conditions as following: consistency of hexamethylendiamine was 15％, at 50°C for 120min and consistency, at 30°C for 45minites, consistency of enzyme was 15mg/mL at 4°C.So the immobilized amount of protein could come to 30.23mg/g membrane and the activity of immobilized MTG come to 16.9U/g membrane.

关键词： transglutaminase Polypropylene Microporous Membranes immobilization

作者: NA Zhiguo WANG xuefei MA Yongqiang QIAN Lei YAN Hongyu

作者单位: Department of Food and Environmental Engineering,Heilongjiang East College,Harbin ,China College of Food Engineering,Harbin University of Commerce,Harbin,China

会议类型: 国际会议

会议名称: 2011 2nd International Conference on Material and Manufacturing Technology(2011第二届材料与制造技术国际会议 ICMMT2011)

会议地点: 厦门

会议语种:英文

页码: 338-344

在线出版日期: 2011-07-08（万方平台首次上网日期，不代表论文的发表时间）