Expression of mineralized tissue associated proteins: Dentin sialoprotein and phosphophoryn in rodent hair follicles
Objective: In this study, we examined the presence and location of DSP/PP proteins during hair follicle development. Methods: Rat PP proteins were detected using immunohistochemical/immunofiuorescent staining. DSPPP mRNAs were detected by in situ hybridization with riboprobes. LacZ expression was detected in mouse tissues using a DSP-PP promoter-driven LUC in transgenic mice. Results: We found that PP proteins and DSP-PP mRNAs are present in rat hair follicles. We also demonstrate that an 8 kb DSP-PP promoter is able to drive lacZ expression in hair follicles. Conclusion: We have firmly established the presence of DSP/PP in mouse and rat hair follicles by immunohistochemical/immunofiuorescent staining, in situ hybridization with riboprobes and transgenic mice studies. The expression of DSP/PP in hair follicles is the first demonstration that major mineralization proteins likely may also contribute to soft tissue development. This finding opens a new avenue for future investigations into the moleculargenetic management of soft tissue development.
Dentin protein expression Dentin sialoprotein Phosphophoryn Hair follicles
Xu-na Tang Ya-qin Zhu Cynthia L Marcelo Helena H. Ritchie
Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Mi Department of General Dentistry, Ninth Peoples Hospital, Shanghai jiao tong University School of Me Department of Surgery, Section of Plastic and Reconstructive Surgery, University of Michigan, Ann Ar Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Mi
国际会议
上海
英文
67-73
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）