Investigation of Supragingival Plaque Microbiota in Different Caries Status of Chinese Preschool Children by Denaturing Gradient Gel Electrophoresis
This study aimed to detect differences in the richness of total supragingival plaque microbiota as well as the species composition of oral streptococci involved in the different stages of dental caries. Forty-five plaque samples were collected from cariesmoderate (CM, 4<dmfs≤6), caries-susceptible (CS, dmfs≥10), and age-matched caries-free children separately. Total DNA was isolated directly from each sample, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses using universal and primers specific for oral streptococci were carried out. Using 16S rDNA PCRDGGE, 34 different species of bacteria were identified in a culture-independent manner and classified into 11 genera according to phylogenetic analysis. Among them, Mitis group streptococci and Campylobacter, which were present in health status, no longer appeared in caries-susceptible samples. In addition, Capnocytophaga, Burkholderia, and Prevotella were found significantly less frequently in the CS group samples (P<0.05), while there were no significant differences among the prevalence of Neisseria, Leptotrichia, Haemophilus, Mutans group streptococci, Corynebacterium, and Actinomyces in the three groups. Further DGGE analysis of rnpB gene amplicons obtained with oral streptococci species-specific primers showed that a total of 23 species of oral streptococci were identified. Streptococcus sanguinis, Streptococcus mitis, and Streptococcus oralis showed a significantly higher prevalence in healthy children (P<0.05), while that of Streptococcus mutans and Streptococcus sobrinus did not vary among the three groups. Overall, these results suggest that supragingival plaque microbiota as a whole undergoes a more complicated shift in the caries process.
Wei Jiang YunTao Jiang ChaoLun Li JingPing Liang
Department of Endodontics and Operative Dentistry, School of Stomatology, Ninth Peoples Hospital, S Department of Periodontology, School of Stomatology, Ninth Peoples Hospital, Shanghai Key Laborator
国际会议
上海
英文
121-131
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）