Effect of human beta-defensin-3 on the proliferation of fibroblasts on periodontally involved root surfaces
Human beta-defensin-3 (HBD-3) has versatile antibacterial activity against oral bacteria and can promote the proliferation of fibroblasts. The goal of the present study was to investigate the effect of HBD-3 on attachment and proliferation of periodontal ligament cells (PDL) onto the periodontitis affected root surfaces. PDL cells were seeded onto healthy and diseased root specimens with scaling and root planing (SRP), SRP & HBD-3 (l00ng/ml), or SRP & HBD-3 (200ng/ml) treatment for 1,3, and 7days incubation. The results showed that HBD-3, especially in the 200ng/mI group, significantly promoted fibroblast attachment and proliferation onto the diseased root surfaces. The cell number in the HBD-3 group was much greater than in the group treated with SRP alone. On day 7, the cells in the HBD-3 were well-spread and formed a network similar to those on the surfaces of the healthy root specimens. These results suggest that HBD-3 could play an important role in antibacterial activity and fibroblast proliferation, thus promoting periodontal regeneration. Meanwhile, HBD-3 might act as a potent regeneration-promoter in infectious diseases.
Periodontitis Human beta-defensin-3 PDL cells Cell proliferation Periodontal regeneration
Haiyan Wang Hisashi Watanabe Mayumi Ogita Shizuko Ichinose Yuichi Izumi
Department of Oral Mucous Diseases, Ninth Peoples Hospital, Shanghai Jiao Tong University School of Section of Periodontology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Instrumental Analysis Research Center for Life Science, Tokyo Medical and Dental University, Tokyo 1
国际会议
上海
英文
159-165
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）