An Ectopic Study of Apatite-Coated Silk Fibroin Scaffolds Seeded with AdBMP-2-Modified Canine bMSCs
(0) The present study was undertaken to evaluate ectopic new bone formation effects of apatite-coated silk fibroin scaffolds (mSS) seeded with adenovirusmediated bone morphogenic protein-2 gene (AdBMP-2) transduced canine bone marrow stromal cells (bMSCs) in nude mice. In this study, bMSCs derived from canine were cultured and transduced with AdBMP-2 enhanced green fluorescent protein gene (AdEGFP) in vitro. Osteogenic differentiation of bMSCs was determined by alkaline phosphatase (ALP) activity analysis, and the transcript levels for BMP-2, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) genes via real-time quantitative PCR (RT-qPCR) analysis. The ectopic bone formation effects of mSS seeded with AdBMP-2-modified bMSCs were evaluated through histological and histomorphological analysis 4, 8 and 12 weeks postoperation in nude mice. ALP activity was statistically increased in AdBMP-2 group, when compared with control groups. The mRNA expression of BMP-2, OPN, OCN and BSP was also statistically upregulated 6, 9 days after AdBMP-2 transduction. Significantly higher bone volume was achieved in AdBMP-2-transduced bMSCs/mSS constructs than that of AdEGFP-transduced bMSCs/mSS or bMSCs/mSS groups at 4, 8 and 12 weeks (P < 0.01). These results demonstrated that mSS seeded with AdBMP-2-transduced canine bMSCs can promote ectopic new bone formation and maturation in nude mice suggesting the potential of this silkscaffold-based tissue-engineered bone for further bone regeneration studies in canine models.
Kaige Lu Ling Xu Lunguo Xia Yilin Zhang Xiuli Zhang David L. Kaplan Xinquan Jiang Fuqiang Zhang
Department of Prosthodontics, College of Stomatology, Ninth Peoples Hospital, School of Medicine, S Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth Peoples Hospital, Schoo Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth Peoples H Department of Biomedical Engineering, Tufts University, Boston, MA 02155, USA Department of Prosthodontics, College of Stomatology, Ninth Peoples Hospital, School of Medicine, S
国际会议
上海
英文
273-288
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）