PROLIFERATION AND OSTEOGENIC DIFFERENTIATION OF HUMAN PERIODONTAL LIGAMENT CELLS ON AKERMANITE AND p-TCP BIOCERAMICS
The purpose of this study was to investigate the effects of akermanite as compared to P-TCP on attachment, proliferation, and osteogenic differentiation of human periodontal ligament cells (hPDLCs). Scanning electron microscopy (SEM) and actin filament labeling were used to reveal attachment and growth of hPDLCs seeded on P-TCP and akermanite ceramic. Cell proliferation was tested by lactic acid production and MTT analysis, while osteogenic differentiation was assayed by alkaline phosphatase (ALP) expression and real-time polymerase chain reaction (PCR) analysis on markers of osteopontin (OPN), dentin matrix acidic phosphoprotein-1 (DMP-1), and osteocalcin (OCN), and further detected by enzyme-linked immunosorbent analysis (ELISA) analysis for OCN expression. Besides, the ions released from akermanite and their effect on hPDLCs was also measured by inductively coupled plasma atomic emission spectroscopy (ICPAES), MTT analysis, ALP expression and real-time PCR analysis. hPDLCs attached well on both ceramics, but showed better spreading on akermanite. hPDLCs proliferated more rapidly on akermanite than P-TCP. Importantly, osteogenic differentiation of hPDLCs was enhanced on akermanite compared to P-TCP. Besides, Ca, Mg and Si ions were released from akermanite, while only Ca ions were released from P-TCP. Moreover, more pronounced proliferation and higher osteogenic gene expression for hPDLCs cultured with akermanite extract were detected as compared to cells cultured on akermanite. Therefore, akermanite ceramic showed an enhanced effect on proliferation and osteogenic differentiation of hPDLCs, which might be attributed to the release of ions containing Ca, Mg and Si from the material. It is suggested that akermanite ceramics may serve as a potential material for periodontal bone regeneration.
Akermanite human periodontal ligament cells bone regeneration.
Lunguo Xia Zhiyuan Zhang Lei Chen Wenjie Zhang Deliang Zeng Xiuli Zhang Jiang Chang Xinquan Jiang
Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth Peoples Hospital, Schoo State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute o Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth Peoples Hospital Affilia
国际会议
上海
英文
1-16
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）