Decreased expression of profilin 2 in oral squamous cell carcinoma and its clinicopathological implications
(0) Profilins are small proteins essential for many normal cellular dynamics and constitute one of the crucial components of actin-based cellular motility. Several recent studies have implicated a role for the profilin (PFN) family in cancer pathogenesis and progression. However, their expression and promising functions are largely unknown in oral squamous cell carcinoma (OSCC). In this study, we analyzed the correlation between PFN1 and PFN2 expression in vitro and in vivo. The protein expression levels were roughly compared between cell lines (HIOEC, HB96) with the employment of mass spectrometry. PFN2 was singled out as one of the significantly downregulated genes in the cancerous HB96 cells. The expression levels of PFN1 and PFN2 in vitro were validated by RT-PCR, real-time PCR and Western blotting. Laser scanning confocal microscopy was used for the first time to assess the localization of PFN2 expression. In subsequent experiments, we observed the relationship between PFN2 expression levels and the proliferation of transfected HB96 cancer cells. VASP, N-WASP and P27 expression was also examined in the PFN2-transfected or non-transfected HB96 cells. In vivo, antigen expression was determined by immunohistochemical analyses in 88 paired tissue specimens. Decreased protein expression was confirmed in cancerous tissues from 88 OSCC patients compared with paracancerous normal mucous epithelia. Tumors with weak PFN2 expression were associated with a significantly worse prognosis than strongly expressed tumours (P<0.001). Other statistical analyses were performed to assess the differences in expression and their clinical and pathological significance. In conclusion, PFN2 can be utilized as both a potential suppressor marker and a prognostic protein for OSCC. The function of PFN2 may be to regulate the NWASP/Arp2/3 signaling pathway.
profilin decreased expression oral squamous cell carcinoma prognosis N-WASP
C.Y.MA C.P. ZHANG L.P.ZHONG H.Y.PAN W.T. CHEN L.Z.WANG O.W.ANDREW T.JI W. HAN
Department of Oral and Maxillofacial Surgery, Ninth Peoples Hospital, College of Stomatology Shangh Department of Oral Pathology, Ninth Peoples Hospital, College of Stomatology, Shanghai Jiao Tong Un Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, National University of Singapore Department of Oral and Maxillofacial Surgery, Ninth Peoples Hospital, College of Stomatology
国际会议
上海
英文
227-237
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）