Increased expression of Toll-like receptor-9 has close relation with tumour cell proliferation in oral squamous cell carcinoma
Objective: Toll-like receptor-9 (TLR-9), a new member of the interleukin-1 receptor super-family, was recently found to have a high level of expression in many carcinoma specimens. The objective of this study was to examine the TLR-9 expression and its role in tumour cell proliferation in oral squamous cell carcinoma. Materials and methods: Western blot and immunonistochemistry were used to detect TLR-9 protein in oral squamous cell carcinoma (OSCC) Tca8113 cell lines and clinical specimens (n = 60). The relationship between TLR-9 expression and clinicopathologic features was analysed. Cell proliferation and inflammatory chemokines secretion were tested by MTT and ELISA methods respectively. Results: Results showed that TLR-9 expression level was higher in OSCC tissues than in paired adjacent normal tissues (P < 0.01), and the expression level of TLR-9 was significantly associated with tumour size (P = 0.001), tumour clinical stage (P = 0.003) and Ki-67 expression (P < 0.01). In vitro results also suggested that stimulation of Tca-8113 cells with TLR-9 agonist CpG-ODN could significantly increase tumour cell proliferation as well as subsequent IL-la and IL-6 secretions (P < 0.01), which could be partially inhibited by usage of antiTLR-9 protein. Conclusions: It was therefore hypothesized that increased expression of TLR-9 may be of great value in assessing the development of OSCC, and could be used as a new target for OSCC prevention and therapy in future.
Toll-like receptor-9fJLR-9 Oral squamous cell carcinoma (OSCC) Ki-67 Cell proliferation Inflammatory chemokines
Ruan Min Zhang Zun Li Siyi Yang Wenjun Wang Lizheng Zhang Chenping
Department of Oral and Maxillofacial Surgery, Affiliated No. 9 Peoples Hospital, Shanghai JiaoTong Department of Stomatology, Affiliated No. 1 Peoples Hospital, College of Medicine, Shanghai Jiao To Department of Oral Pathology, Affiliated No. 9 Peoples Hospital, Shanghai JiaoTong University Schoo
国际会议
上海
英文
327-334
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）