Factors Influencing Formation of the Plantlet from Microspore Embryoid in Pakchoi (Brassica Campestris ssp. Chinensis L)
The aim of this study was to optimize culture system for plantlet regeneration from embryoids in Pakchoi. Isolated microspore culture was employed in 10 hybrid varieties. The effects of genotype, seal materials and concentration of different additives to the medium on the formation of plantlet were studied by setting up different treatments. Results indicated that genotypes significantly affected the frequency of plantlet regeneration. The optimum medium was MS+ 3%sucrose+1.0%agar+0.1gLactivated charcoal; Ventilate well Sealed film was beneficial to plantlet regeneration.
Pakchoi microspore culture embryoid plantlet regeneration
Liu Xueli Yang Shuo Fu Ying Feng Hui
College of Horticulture, Shenyang Agricultural University
国际会议
The 3rd Conference on Key Technology of Horticulture(第三届园艺关键技术学术会议 CKTH 2011)
沈阳
英文
257-261
2011-12-17(万方平台首次上网日期,不代表论文的发表时间)