LYMPHOCYTES FAVOR THE ATTACHMENT OF CD11A-TARGETED MICROBUBBLES WITH ENDOTHELIAL CELLS IN VITRO
Ultrasound molecular imaging may provide new insights into the early detection and diagnose of diseases, such as atherosclerosis, thrombosis as well as inflammations by the attachment of various targeted contrast agents with disordered endothelium. However, molecular imaging of disordered endothelium in large and middle-sized arteries by site-specific accumulation of contrast agents is still difficult to achieve due to wall shear stress conditions in these vessels. Here, we provided an alternative strategy to improve the attachment of targeted contrast agents with endothelium with the aid of lymphocytes. Given the fact that lymphocytes are naturally equipped to adhere selectively to endothelium and to resist physiologic shear stresses in large- and middle-sized arteries, in this study, the targeted microbubbles were prepared by conjugating anti-CDlla antibodies to the biotinylated microbubbles through biotin-avidin linkage. The resulting targeted microbubbles were able to bind with TNF-a-stimulated lymphocytes, which could also adhere to murine endothelial bEnd.3 cells at same time. The number of adhered microbubbles increased in concomitant with elevation of TNF-a concentration, with mean 4±4, 7±4, 21±9 and 103 ± 13 microbubbles per view field (200 ×) for 0.4, 2, 10 and 50 ng/ml of TNF-a stimulation, respectively. In contrast, fewer targeted microbubbles adhered to the TNF-a-stimulated endothelial cells without assistance of lymphocytes. These findings suggested that lymphocytes might be useful as a vehicle of targeted microbubbles to increase the site-specific accumulation of targeted ultrasound contrast agent and have potential application to ultrasound molecular imaging of cardiovascular diseases.
Targeted microbubbles Lymphocyte endothelial cells Adhesion
Wei YANG FeiYAN Juan-juan CHEN Hai-rong ZHENG
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055 Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016 Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055 Key Lab of
国际会议
深圳
英文
314-316
2011-12-09（万方平台首次上网日期，不代表论文的发表时间）