Preparation and Evaluation of Antioxidative Peptide Fractions from Mytilus coruscus Protein

摘要：

Using·OH scavenging test, an efficient method had been developed to acquire protein hydrolysate (MMH) from Mytilus coruscus by an orthogonal L9(34) test. The optimal enzymolysis parameters were enzymolysis time 3 h, temperature 60亷, solid-liquid ratio 1:2 and enzyme dose 3％. Enzymolysis temperature and solid-liquid ratio showed significant effects (ANOVA p<0.05) on the hydrolysate preparation. Based on the molecular weight (MW), MMH-I (10<MW<3 kDa) and MMH-II (MW<3 kDa) were fractionated from MMH by ultrafiltration membrane system, and MMH-I showed higher antioxidant activity in the two fractions. F21 subfraction with stronger antioxidant activity was obtained from MMH-I by ion-exchange and gel filtration chromatography. The .OH scavenging activity of F21 was 6.12％ at the concentration of 2.0 mg/mL.

关键词： Mytilus coruscus antioxidant activity peptide fractions

作者: Jia Hui Ma Bin Wang Yin Feng Xu Li Li You Le Qu Shang Gui Deng

作者单位: School of Food and Pharmacy, Zhejiang Ocean University, Qixiangtai Road 51, Zhoushan 316004,PR China

会议类型: 国际会议

会议名称: 2011 International Conference on Advanced Materials and Engineering Materials(2011先进材料与工程材料国际会议 ICAMEM 2011)

会议地点: 沈阳

会议语种:英文

页码: 338-342

在线出版日期: 2011-11-22（万方平台首次上网日期，不代表论文的发表时间）